AIF (AIFM1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, IP, Simple Western, WB
Recommend Dilution	Immunohistochemistry, Immunoprecipitation: 1:50-1:200, Western Blot: 1:1000-1:2000~, Immunohistochemistry-Paraffin: 1:1000-1:5000~, Immunohistochemistry-Frozen: 1:1000-1:5000, Simple Western: 1:500, Knockout Validated
Reactivity	Human, Mouse, Rat, Bovine, Canine
Host	Rabbit
Clonality	Polyclonal
Immunogen	(aa 151-170); human
Formulation	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	apoptosis inducing factor, mitochondria associated 1
Database Link	NP_665811 Entrez Gene 26926 MouseEntrez Gene 83533 RatEntrez Gene 9131 Human UniProt ID O95831
Background	AIF (apoptosis-inducing factor) was initially discovered as a protein involved in caspase-independent cell death. It is now known that AIF has both vital and lethal functions (reviewed in Modjtahedi et al, 2006). In healthy cells, AIF is a flavoprotein present in the mitochondria where it has vital roles in cellular redox metabolism and mitochondrial bioenergetics. In many models of apoptosis, AIF is released from the mitochondria during mitochondrial outer membrane permeabilization along with other mitochondrial proteins. Upon release, AIF translocates first to the cytosol and then to the nucleus where it induces chromatin condensation and DNA degradation. Although several lines of evidence suggest that AIF is a main mediator of capsase-independent cell death, the mechanisms regulating AIF pro-apoptotic function remain to be fully elucidatied and may depend on the cell type and type of apoptotic stimuli. Human AIF is transcribed from a nuclear gene located on the X chromosome and translated in the cytoplasm to a precursor protein of 613 amino acids (aa) which corresponds to ~67 kDa. The precursor protein is imported into the mitochonria by mitochondrial localization sequences located within the N-terminal prodomain of AIF. Once inside the mitochondria, the prodomain is cleaved giving rise to a mature AIF form of ~57 kDa. AIF isoforms generated from a single AIF gene have been identified, including AIF short isoform 2 (324 aa protein; GenBank no. AAY84739.1) and AIF short isoform 3 (237 aa protein; GenBank noAAY84741.1 (reviewed in Delttre et al, 2006). The generation of multiple isoforms from a common gene is an evolutionary mechanism that increases protein diversiity in eukaryotes. Regulating gene expression through the production of multiple isoforms from a single gene is thought to play a major role in the control of apoptosis and other forms of programmed cell death. It recognizes AIF and AIF isoforms containing the peptide immunogen sequence, RARDPGARVLIVSEDPELP.
Synonyms	AIF; CMT2D; CMTX4; COWCK; COXPD6; NADMR; NAMSD; PDCD8
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Apoptosis

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