BAG3 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, IP, Simple Western, WB
Recommend Dilution	Immunohistochemistry, Immunohistochemistry-Paraffin: 1:1000-1:5000~, Immunohistochemistry-Frozen: 1:1000-1:5000, Simple Western: 1:100, Immunocytochemistry/ Immunofluorescence: 1:200, Immunoprecipitation: 1:50-1:200, Western Blot: 1:1000-1:2000~
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A recombinant protein fragment corresponding to the C-terminal 196 amino acids of human BAG-3. Bag-3; full length-gel=GST-RP; human
Formulation	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	BCL2 associated athanogene 3
Database Link	NP_004272 Entrez Gene 29810 MouseEntrez Gene 9531 Human UniProt ID O95817
Background	The BAG proteins are a family of chaperone regulators that modulate a number of diverse processes including proliferation, survival, stress responses, tumorigenesis, neuronal differentiation, growth arrest and apoptosis (reviewed Takayama and Reed, 2001; Doong et al, 2002, and Doukhanina et al. 2006). BAG proteins have been characterized as co-chaperones and interact with the chaperone heat shock proteins 70, both constitutive Hsc70 and inducible Hsp70. BAG proteins bind through their BAG domain to the ATPase domain of Hsc70/Hsp70, and can modulate either positively or negatively the functions of the Hsc70/Hsp70 chaperone proteins. The BAG domain has been shown to contribute to the anti-apoptotic activity of BAG- family proteins. The anti-apoptotic activities of BAG-family proteins may be dependent on their interactions with Hsc70/Asp70 and/or binding to Bcl-2. In addition to the conserved BAG domain, BAG-family proteins also contain additional domains which enable them to interact with specific target proteins or to target them to specific locations within cells. The BAG family contains at least six family members, including BAG-1 and its various isoforms [including BAG-1S , BAG-1M (RAP46/HAP46), and BAG-1L, BAG2, BAG3 (CAIR-1; Bis,), BAG4 (SODD), BAG5 and BAG6 (Scythe, BAT3). The following amino acids (aa) lengths and molecular weights (kDa) have been described for human BAG proteins (reviewed in Takayama et al, 2001 and Doong et al, 2002): BAG-1 (230 aa., 34 kDa), BAG-1S (219 aa, 29 kDa), BAG-1M (274 aa, 46 kDa), BAG-1L (345 aa, 52 kDa), BAG-2 [212 aa; 24 kDa (Arndt et al. 2005)], BAG-3 (575 aa, 74 kDa), BAG-4 (456 aa; 60 kDa), BAG-5 ([442 aa; 51 kDa (kalia et al. 2004)], and BAG-6 (1129 aa; 150 kDa). IMG-5668 recognizes both BAG-3; BAG-3 migrates at ~74-80 kDa on SDS-PAGE.
Synonyms	BAG-3; BIS; CAIR-1; MFM6
Note	1. Increased BAG-3 expression has been described in pancretic cancer compared to normal pancreas, Detectible BAG-3 expression has also been described inserveral pancreatric cancer cell lines. Please see Liao et al (2001) for details.2. In normal organs, BAG-3 protein has been found to be abundantly expressed in muscle. Please see Homma et al for details.
Reference Data
Protein Families	Druggable Genome

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