Lin28 (LIN28A) Mouse Monoclonal Antibody [Clone ID: 2C1-F9-A2]

Specifications

Product Data
Clone Name	2C1-F9-A2
Applications	WB
Recommend Dilution	WB: 1:1000
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	The immunogen for LIN28A antibody: purified recombinant human LIN28A protein fragments expressed in E.coli
Formulation	Purified mouse monoclonal in PBS(pH 7.4)containing with 0.02% sodium azide and 50% glycerol.
Purification	Affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	26 kDa
Gene Name	lin-28 homolog A
Database Link	NP_078950 Entrez Gene 83557 MouseEntrez Gene 79727 Human UniProt ID Q9H9Z2
Background	Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis.Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and,indirectly,in stabilizing the mRNAs.Binds IGF2 mRNA,MYOD1 mRNA,ARBP/36B4 ribosomal protein mRNA and its own mRNA.Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression By similarity.Acts as a suppressor of microRNA(miRNA)biogenesis by specifically binding the precursor let-7(pre-let-7),a miRNA precursor.Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase,leading to the terminal uridylation of pre-let-7.Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation.Degradation of pre-let-7 in embryonic stem(ES)cells contributes to the maintenance of ES cells.In contrast,LIN28A down-regulation in neural stem cells by miR-125,allows the processing of pre-let-7.Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7.Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
Synonyms	CSDD1; LIN-28; lin-28A; LIN28; ZCCHC1
Reference Data
Protein Families	Transcription Factors

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.