HMG1 (HMGB1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	WB: 1 µg/mL; IHC: 1-2 µg/mL; IF: 20 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse, and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	HMGB1 antibody was raised against a 19 amino acid peptide near the center of human HMGB1.
Specificity	HMGB1 antibody is human, mouse and rat reactive.
Formulation	PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	HMGB1 antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	Predicted: 24 kDa; Observed: 24 kDa
Gene Name	high mobility group box 1
Database Link	NP_002119 Entrez Gene 15289 MouseEntrez Gene 25459 RatEntrez Gene 3146 Human UniProt ID P09429
Background	The high-mobility group box 1 (HMGB1) protein was originally identified as a highly conserved nuclear DNA-binding protein that participates in DNA replication, repair and transcriptional regulation of gene expression (1,2). However, HMGB1 has more recently emerged as an extracellularly released mediator of inflammation and repair responses in lipopolysaccharide (LPS)-induced endotoxemia and sepsis (3). HMGB1 has also been suggested to induce secretion of pro-inflammatory cytokines and chemokines by inducing NF-kB signaling downstream of Toll-like receptor (TLR) 2 and TLR4 activation (4).
Synonyms	HMG1; HMG3; SBP-1
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways	Base excision repair

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