beta Actin (ACTB) Mouse Monoclonal Antibody [Clone ID: 10B7]

Specifications

Product Data
Clone Name	10B7
Applications	ELISA, WB
Recommend Dilution	b-actin antibody can be used for the detection of b-actin by Western blot at 0.5 - 2 µg/mL. Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Reactivity	Human, Mouse, Rat, Rabbit, Zebrafish, Chicken, Drosophila
Host	Mouse
Clonality	Monoclonal
Immunogen	b-actin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human b-actin.
Specificity	beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.
Formulation	beta-Actin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	beta-Actin Antibody is Protein G purified.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	Predicted: 42 kDa; Observed: 44 kDa
Gene Name	actin, beta
Database Link	AAH02409 Entrez Gene 60 Human UniProt ID P60709
Background	Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Synonyms	Beta-actin; PS1TP5BP1
Note	b-actin antibody can be used for the detection of b-actin by Western blot at 0.5 - 2 µg/mL.
Reference Data

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