PD1 (PDCD1) Mouse Monoclonal Antibody [Clone ID: 4D6]

Specifications

Product Data
Clone Name	4D6
Applications	ELISA, IF, IHC, WB
Recommend Dilution	WB: 0.25-0.5µg/mL. IHC starting at 5µg/mL. IF start at 20µg/mL.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	PD-1 antibody was raised against the extracellular domain of human PD-1.
Formulation	PD-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	PD-1 Antibody is supplied as protein A purified IgG1.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	Predicted: 32 kDa; Observed: 38 kDa
Gene Name	programmed cell death 1
Database Link	NP_005009 Entrez Gene 5133 Human UniProt ID Q15116
Background	Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD-1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. PD-1 is thus one of a growing number of immune checkpoint proteins.
Synonyms	CD279; hPD-1; hPD-l; PD1; SLEB2
Note	PD-1 antibody can be used for detection of PD-1 by Western blot at 0.25 - 0.5 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Reference Data

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