B7-1 (CD80) Mouse Monoclonal Antibody [Clone ID: 8G12]

Specifications

Product Data
Clone Name	8G12
Applications	FC, IF, IHC
Recommend Dilution	IHC starting at 2-5µg/mL. IF start at 5µg/mL. FC: 10µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	CD80 antibody was raised against the extracellular domain of human CD80.
Formulation	CD80 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	CD80 Antibody is supplied as protein A purified IgG2b.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	Predicted: 32 kDa; Observed: 50 kDa
Gene Name	CD80 molecule
Database Link	NP_005182 Entrez Gene 941 Human UniProt ID P33681
Background	CD80 Antibody: CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
Synonyms	B7; BB1; CD28LG; CD28LG1; LAB7
Note	CD80 antibody can be used for immunohistochemistry starting at 2 - 5 µg/mL. For immunofluorescence start at 5 µg/mL. Flow cytometry at 10 µg/ml.
Reference Data

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