IL2 Receptor alpha (IL2RA) Mouse Monoclonal Antibody [Clone ID: 143-13]

Specifications

Product Data
Clone Name	143-13
Applications	IHC
Recommend Dilution	Tested for immunohistochemistry (IHC); has been described to work in FACS. Approximate working dilution for IHC: Frozen sections: 3µg/ml (1:130) Paraffin sections: not tested Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Stimulated human leukocytes.
Specificity	Human: CD25 Other: not tested. Epitope: The antigen is CD25, a 55kD glycoprotein. The antibody recognizes epitope region C of the IL-2 receptor complex. Distribution: Isolated cells: The antibody stains less than 5% of resting human peripheral blood mononuclear cells in flow cytometry. Tissue sections: Scattered large and small lymphocytes in germinal centers, in follicle mantle, and T-zone.
Formulation	Affinity purified, lyophilized Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.4mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA), and 0.09% sodium azide as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: 1 year at 4° - 8°C. Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing.
Gene Name	interleukin 2 receptor subunit alpha
Database Link	Entrez Gene 3559 Human UniProt ID P01589
Background	Monoclonal antibody 143-13 recognizes the CD25 antigen.CD25, the IL-2 receptor alpha chain, associates with CD122 and the common gamma chain CD132 to form the IL-2 receptor complex. It is expressed on activated T and B cells and on monocytes.
Synonyms	CD25; IDDM10; IL-2-RA; IL2-RA; IL2R; p55; TCGFR
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.