CD3E Mouse Monoclonal Antibody [Clone ID: UCHT-1]

Specifications

Product Data
Clone Name	UCHT-1
Applications	IHC
Recommend Dilution	Tested for immunohistochemistry (IHC); has been described to work in FACS. Approximate working dilution for IHC: Frozen sections: 0.2µg/ml (1:1000) Paraffin sections: not determined Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human thymocytes followed by Sezary T cells.
Specificity	Human: CD3 Other: not tested. Epitope: The antigen is CD3. The epitope is located on the ε chain. Distribution: Isolated cells: The antibody stains approximately 60-90% of human peripheral blood mononuclear cells in flow cytometry.
Formulation	Affinity purified, lyophilized Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.2mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA), and 0.09% sodium azide as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: 1 year at 4° - 8°C. Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing.
Gene Name	CD3e molecule
Database Link	Entrez Gene 916 Human UniProt ID P07766
Background	Monoclonal antibody UCHT-1 recognizes the CD3 antigen on mature human T-cells. The CD3 antigen is a protein complex composed of four distinct chains (CD3γ, CD3δ and two CD3ε) that associate with the T cell receptor (TCR) and the ζ-chain to generate an activation signal in T lymphocytes. The TCR, ζ-chain and CD3 molecules together comprise the TCR complex. The CD3γ, CD3δ and CD3ε chains are highly related cell surface proteins of the immunoglobulin superfamily containing a single extracellular immunoglobulin domain.
Synonyms	CD3-epsilon; FLJ18683; T3E; TCRE
Reference Data

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