CD69 Monoclonal Antibody [Clone ID: FN50 Biotin]

Specifications

Product Data
Clone Name	FN50 Biotin
Applications	IHC
Recommend Dilution	Tested for immunohistochemistry (IHC); has been described to work in FACS. Approximate working dilution for IHC: Frozen sections: 2 - 4µg/ml (1:50 - 1:100) Paraffin sections: does not react on routinely processed paraffin sections. Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil
Reactivity	Human
Clonality	Monoclonal
Specificity	Human: CD69 Other: not tested. Epitope: The antigen is CD69. The epitope is a 53kD molecule. Under reducing conditions it showed two bands of 29 and 32 kD. Distribution: Isolated cells: > 90% of activated human peripheral blood lymphocytes. Tissue sections: In tonsil the antigen is strongly expressed on CD4+ T-cells in the light zone of the follicle centre, on extrafollicular T-cells, and weakly on mantle zone B-cells. In the spleen, the antigen is found on CD4+ T-cells in the middle of the marginal zone, while in thymus most of the medullary thymocytes are strongly positive.
Formulation	Affinity purified, biotinylated, lyophilized Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.2mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA), and 0.05% (v/v) Kathon CG as a preservative.
Concentration	N/A
Conjugation	Biotin
Storage Condition	Original vial: 1 year at 4° - 8°C. Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing.
Gene Name	CD69 molecule
Database Link	Entrez Gene 969 Human UniProt ID Q07108
Synonyms	AIM; BL-AC/P26; CLEC2C; EA1; GP32/28; Leu-23; MLR-3
Reference Data

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