Cd163 Mouse Monoclonal Antibody [Clone ID: ED2]

Specifications

Product Data
Clone Name	ED2
Applications	IHC
Recommend Dilution	Tested for immunohistochemistry (IHC); has been described to work in FACS, immunprecipitation. Approximate working dilution for IHC: Frozen sections: 1µg/ml (1:1000) Paraffin sections: 20µg/ml (1:50 Proteinase K pretreatment for antigen retrieval is recommended Optimal dilutions should be determined by the end user. Suggested positive control: Rat spleen.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat spleen cells.
Specificity	Rat: macrophages. Other species: not tested Epitope: The antigen has been described as CD163, epitope not further characterized. Distribution: Isolated cells: Monocytes and dendritic cells harvested from spleen and lymph nodes, and granulocytes harvested from the peritoneal cavity are negative with ED 2.
Formulation	Affinity purified, liquid. Supplied as a 0.25ml solution. This stock solution contains 1mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), no stabilizer and 0.09% sodium azide as a preservative.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing.
Gene Name	CD163 molecule
Database Link	Entrez Gene 312701 Rat UniProt ID D3Z9U2
Background	Monoclonal antibody ED2 reacts with a membrane antigen (175, 160 and 95kDa) on resident rat macrophages. Monocytes, dendritic cells (spleen and lymph node), peritoneal granulocytes and other cell types are negative. ED2 discriminates between thymic cortical (positive for ED2) and medullary (negative for ED2) macrophages. It is useful to stain synovial lining cells and Kupffer cells in the liver. The antigen has recently been shown to be identical with CD163 (Polfliet, M.M.J. et al [2002], PhD Thesis, Vrije Universiteit, Amsterdam).
Synonyms	M130; MM130
Reference Data

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