ALB Mouse Monoclonal Antibody [Clone ID: PAL17]

Specifications

Product Data
Clone Name	PAL17
Applications	ELISA, IHC, WB
Recommend Dilution	Tested for immunohistochemistry (IHC), Western Blot and ELISA, other applications not yet tested. Approximate working dilutions: IHC, frozen sections: 0.1µg/ml (1:4000) IHC, paraffin sections: 0.5µg/ml (1:800); microwave pretreatment for antigen retrieval is recommended.
Reactivity	Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Porcine kidney extract.
Specificity	Porcine Albumin Other species: Western Blot analysis indicates no detectable reactivity with human, horse, rat, goat, bovine, sheep or donkey albumin. Titration of the antibody against purified porcine, human or rabbit albumin confirms minimal cross-reactivity. Epitope: Immunoprecipitation and subsequent identification by nanoLC- ESI-MS/MS identified the antigen as porcine albumin. The epitope has not been further characterized. Distribution: Tissue sections: Hepatocytes of the liver are stained positive, and albumin in the blood vessels of all organs.
Formulation	Affinity purified from cell culture supernatant, liquid. This stock solution contains 0.4mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA) as a stabilizer and 0.05% (v/v) Kathon CG as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: 1 year at 4° - 8°C. Avoid repeated thawing and freezing of the reconstituted antibody.
Database Link	UniProt ID P08835
Background	Monoclonal antibody PAL17 reacts specifically with pig albumin, not with albumin from other mammalian species. Albumin has been listed as a stem cell marker for hepatocytes by the U.S. National Institutes of Health. Serum albumin is a globular non-glycosylated protein of 65-70kDa molecular weight, well water-soluble and heat-intolerant. It mainly functions as a regulator of colloidal osmotic pressure and as a transport protein for molecules that are poorly water-soluble, and for zinc. Serum albumin is the most abundant of all plasma proteins. Albumin consists of three homologous domains that mainly contain alpha-helices, porcine albumin contains seventeen disulfide bonds altogether. This antibody was produced serum-free, without fetal calf serum.
Synonyms	albumin; DKFZp779N1935; PRO0883; PRO0903; PRO1341
Reference Data

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