HBB Mouse Monoclonal Antibody [Clone ID: PLA114]

Specifications

Product Data
Clone Name	PLA114
Applications	ELISA, IHC
Recommend Dilution	Tested for immunohistochemistry (IHC) and Western Blot, other applications not yet tested. Approximate working dilutions: IHC, frozen sections: 0.2µg/ml (1:2000) IHC, paraffin sections: 10µg/ml (1:40) Western Blot: 0.25µg/ml (1:2000) Optimal dilutions should be determined by the end user.
Reactivity	Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Porcine liver extract.
Specificity	Pig: Erythrocytes, plasma Other species: not tested Epitope: Immunoprecipitation and subsequent identification by nanoLC- ESI-MS/MS identified the antigen as hemoglobin beta chain. The epitope has not been further characterized. Distribution: Tissue sections: In paraffin sections the antibody stains all erythrocytes whereas in frozen sections the antibody yields a picture typical of a molecule leaking out of blood vessels and diffusing into surrounding tissue.
Formulation	Affinity purified from cell culture supernatant, lyophilized. Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.4mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA) as a stabilizer and 0.05% (v/v) Kathon CG as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: 1 year at 4° - 8°C. Avoid repeated thawing and freezing of the reconstituted antibody.
Database Link	UniProt ID P02067
Background	Monoclonal antibody PLA114 is specifically directed against the porcine hemoglobin beta subunit. Hemoglobin contains four subunits, of which two are identical. They form the α2β2 scaffold that holds the iron-containing heme group which is responsible for oxygen transport by red blood cells. Each subunit has a molecular weight of about 16kDa. In humans, the beta-globin gene is activated around the time of birth, replacing embryonic and fetal globin genes. In humans, mutations in the adult β-globin gene cause β-thalassemia and sickle cell disease (SCD). β-Thalassemia results from a reduced production of β-globin while SCD is caused by a mutation that results in an amino acid substitution of adult β-globin. Hemoglobin tetramers bearing this mutation polymerize inside red blood cells and distort them into a characteristic crescent-shaped appearance. These rigid sickle cells are more prone to lysis and tend to occlude blood flow in the microvasculature, causing severe physiological problems. This antibody was produced serum-free, without fetal calf serum.
Synonyms	beta-globin; CD113t-C; HBD
Reference Data

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