Corticotropin Releasing Factor (CRH) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	This antibody has been tested and validated in ELISA against Corticotropin Releasing Factor. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide H-Ser-Glu-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr- Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Ala-Arg-Ala-Glu-Gln- Leu-Ala-Gln-Gln-Ala-His-Ser-Asn-Arg-Lys-Leu-Met-Glu-Ile-Ile-NH2 coupled to carrier protein.
Formulation	This polyclonal antibody is supplied as a lyophilized powder. The powder should be rehydrated with 50ml of RIA buffer. Upon reconstitution to 50ml total volume, the solution contains 0.1M sodium phosphate buffer (pH 7.4), 0.05M NaCl, 0.1% BSA, 0.01% NaN3, and 0.1% Triton X-100. Store at 4° - 8°C. This should ensure antibody stability for approximately one month.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
Gene Name	corticotropin releasing hormone
Database Link	Entrez Gene 1392 Human UniProt ID P06850
Background	Corticotropin-Releasing Factor (CRF, also known as Corticoliberin) is a 41 amino acid peptide hormone derived from the 196-amino acid preprohormone. It is secreted in the hypothalamus in response to stress, and stimulates both the synthesis and the secretion of adrenocorticotropic hormone (ACTH). CRF is also synthesized in peripheral tissues and placenta and it is known to suppress appetite and increase subjective feelings of anxiety. This antibody was generated by immunization of rabbits with CRF coupled to a carrier protein.
Synonyms	CRF
Reference Data

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