Endothelin 1 (EDN1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	This antibody has been tested and validated in ELISA against Endothelin-1 and in immunohistochemistry (IHC). Other applications like FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
Reactivity	Bovine, Human, Mouse, Porcine, Rat, Dog
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide H-Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys- Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp-OH (Disulfide bonds between Cys1 and Cys15/Cys3 and Cys11) coupled to carrier protein.
Formulation	This polyclonal antibody is supplied as a lyophilized powder. The powder should be rehydrated with 50ml of RIA buffer. Upon reconstitution to 50ml total volume, the solution contains 0.1M sodium phosphate buffer (pH 7.4), 0.05M NaCl, 0.1% BSA, 0.01% NaN3, and 0.1% Triton X-100. Store at 4° - 8°C. This should ensure antibody stability for approximately one month.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
Gene Name	endothelin 1
Database Link	Entrez Gene 1906 Human UniProt ID P05305
Background	Endothelin-1 is a potent vasoconstrictor produced by vascular endothelial cells. It plays an important role in regulation of cardiovascular functions. The binding of Endothelin-1 to its G-protein coupled receptor triggers a signal transduction cascade leading to activation of the protein kinase C (PKC). PKC inhibitor potentially reverses the up-regulation of Endothelin-1. The enhanced Endothelin-1 expression associated with the activation of PKC occurs in early diabetes. This antibody was generated by immunization of rabbits with Endothelin-1 coupled to a carrier protein.
Synonyms	ET1; HDLCQ7; PPET1; Preproendothelin-1
Reference Data

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