Orexin (HCRT) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	This antibody has been tested and validated in ELISA against Orexin A. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
Reactivity	Bovine, Canine, Human, Mouse, Porcine, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide Pyr-Pro-Leu-Pro-Asp-Cys-Cys-Arg-Gln-Lys-Thr-Cys- Ser-Cys-Arg-Leu-Tyr-Glu-Leu-Leu-His-Gly-Ala-Gly-Asn-His-Ala-Ala-Gly- Ile-Leu-Thr-Leu-NH2, (Disulfide bonds between Cys6 and Cys12/Cys7 and Cys14), coupled to carrier protein.
Formulation	Protein A affinity purified from antiserum, lyophilized, packaged under nitrogen. Reconstitute by adding 0.2ml distilled water. This stock solution contains 2mg/ml IgG, phosphate buffer saline pH 7.4 (PBS), and 0.02% (w/v) Thimerosal as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
Gene Name	hypocretin (orexin) neuropeptide precursor
Database Link	Entrez Gene 3060 Human UniProt ID O43612
Background	Orexin A is a 33 amino acid neuropeptide that includes an N-terminal pyroglutamyl residue, C-terminal amidation, and two intramolecular disulfide bridges between Cys 6 – Cys12, and between Cys7 – Cys14. Orexins (Orexin A and Orexin B) are produced by proteolytic cleavage of prepro-orexin. They were so named for their stimulatory role in feeding behavior, but also play important functions in the organism's energy homeostasis and sleep cycles. They exert their effects through G-protein coupled receptors. This antibody was generated by immunization of rabbits with Orexin A coupled to a carrier protein.
Synonyms	Hypocretin; NRCLP1; orexin; OX; PPORX; PPOX
Reference Data

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