Insulin (INS) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	This antibody has been tested and validated in ELISA against C-peptide. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide H-Glu-Ala-Glu-Asp-Leu-Gln-Val-Gly-Gln-Val-Glu- Leu- Gly-Gly-Gly-Pro-Gly-Ala-Gly-Ser-Leu-Gln-Pro-Leu-Ala-Leu- Glu-Gly-Ser- Leu-Gln-OH coupled to a carrier protein.
Formulation	Protein A affinity purified from antiserum, lyophilized, packaged under nitrogen. Reconstitute by adding 0.2ml distilled water. This stock solution contains 2mg/ml IgG, phosphate buffer saline pH 7.4 (PBS), and 0.02% (w/v) Thimerosal as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
Gene Name	insulin
Database Link	Entrez Gene 3630 Human UniProt ID P01308
Background	The connecting peptide, or C-peptide of human proinsulin, is a 31-amino-acid polypeptide that connects insulin's A-chain to its B-chain. In the context of diabetes or hypoglycemia, a measurement of C-peptide blood serum levels can be used to distinguish between different conditions with similar clinical features. In normal tissues, the antigen is found in pancreaticislet β-cells. C-peptide has been found to be a bioactive peptide in its own right, with effectson microvascular blood flow and tissue health. This antibody was generated by immunization of rabbits with C-peptide coupled to a carrier protein.
Synonyms	ILPR; insulin; IRDN; OTTHUMP00000011162; OTTHUMP00000196038; proinsulin
Reference Data

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