Endothelin 1 (EDN1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	This antibody has been tested and validated in ELISA against Endothelin-1. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
Reactivity	Bovine, Human, Mouse, Porcine, Rat, Dog
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide H-Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys- Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp-OH, (Disulfide bonds between Cys1 and Cys15/Cys3 and Cys11) coupled to carrier protein.
Formulation	Protein A affinity purified from antiserum, lyophilized, packaged under nitrogen. Reconstitute by adding 0.2ml distilled water. This stock solution contains 2mg/ml IgG, phosphate buffer saline pH 7.4 (PBS), and 0.02% (w/v) Thimerosal as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
Gene Name	endothelin 1
Database Link	Entrez Gene 1906 Human UniProt ID P05305
Background	Endothelin (ET-1) is a 21 amino acid peptide, also known as preproendothelin-1 (PPET1), that is produced by the vascular endothelium from a 39 amino acid precursor, big ET-1, through the actions of an endothelin converting enzyme (ECE) found on the endothelial cell membrane. ET-1 is involved in regulation of cardiovascular functions where it has been implicated in the pathogenesis of hypertension, coronary vasospasm, and heart failure. It is also known to causes fibrosis of the vascular cells as well as to stimulate production of reactive oxygen species. This antibody was generated by immunization of rabbits with Endothelin-1 coupled to a carrier protein.
Synonyms	ET1; HDLCQ7; PPET1; Preproendothelin-1
Reference Data

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