Endothelin 1 (EDN1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	This antibody has been tested and validated in ELISA against Big Endothelin-1. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide H-Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys- Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp-Val-Asn-Thr-Pro-Glu-His-Val- Val-Pro-Tyr-Gly-Leu-Gly-Ser-Pro-Arg-Ser-OH (Disulfide bridge: 1 - 15 and 3 - 11) coupled to carrier protein.
Formulation	This polyclonal antibody is supplied as a lyophilized powder. The powder should be rehydrated with 50ml of RIA buffer. Upon reconstitution to 50ml total volume, the solution contains 0.1M sodium phosphate buffer (pH 7.4), 0.05M NaCl, 0.1% BSA, 0.01% NaN3, and 0.1% Triton X-100. Store at 4° - 8°C. This should ensure antibody stability for approximately one month.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
Gene Name	endothelin 1
Database Link	Entrez Gene 1906 Human UniProt ID P05305
Background	Big Endothelin-1 (Big ET), is a 38-amino-acid peptide and biological precursor of the potent vasoconstrictor Endothelin-1. It is cleaved to yield endothelin-1 and the C-terminal fragment (22-38) via the activity of a membrane-bound metalloproteinase called endothelin-converting enzyme (ECE). Endothelin is produced by several cell types including vascular endothelial cells, Mesangial cells and kidney cells. Big Endothelin-1 can be hydrolyzed by chymase to generate endothelin 1 (1-21) in vitro. This antibody was generated by immunization of rabbits with Big Endothelin-1 coupled to a carrier protein.
Synonyms	ET1; HDLCQ7; PPET1; Preproendothelin-1
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.