PAR-2 amide Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	This antibody has been tested and validated in ELISA against PAR-2 (1- 6). Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide H-Ser-Leu-Ile-Gly-Arg-Leu-NH2 coupled to a carrier protein.
Formulation	Protein A affinity purified from antiserum, lyophilized, packaged under nitrogen. Reconstitute by adding 0.2ml distilled water. This stock solution contains 2mg/ml IgG, phosphate buffer saline pH 7.4 (PBS), and 0.02% (w/v) Thimerosal as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
Background	Protease activated receptor 2 (PAR-2) is a proteolytically cleaved receptor which stimulates the increase of cytosolic Ca2+. SLIGKV-NH2 corresponds to the tethered ligand sequence of human PAR-2. PAR-2 (1-6) has been used as a proteinase-activated receptor 2 agonist to induce the expression of Hh and Hippo genes in Hek293A cells. This antibody was generated by immunization of rabbits with PAR-2 (1-6) coupled to a carrier protein.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.