spike protein Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB,1:500 - 1:2000
Reactivity	Human coronavirus NL63
Modifications	Unmodified
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant protein of HCoV-NL63 Spike S2.
Formulation	Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Concentration	lot specific
Purification	Affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20℃. Avoid freeze / thaw cycles.
Predicted Protein Size	149kDa
Database Link	UniProt ID Q6Q1S2
Background	S1 region attaches the virion to the cell membrane by interacting with host ACE2, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Reference Data

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