METTL4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	WB,1:1000 - 1:2000 IHC,1:50 - 1:200 IF,1:50 - 1:200
Reactivity	Human, Mouse, Rat
Modifications	Unmodified
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human METTL4 (NP_073751.3).
Formulation	Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Concentration	lot specific
Purification	Affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20℃. Avoid freeze / thaw cycles.
Predicted Protein Size	54kDa
Gene Name	methyltransferase like 4
Database Link	Entrez Gene 64863 Human UniProt ID Q8N3J2
Background	N(6-adenine-specific methyltransferase that can methylate both RNAs and DNA. Acts as a N(6-adenine-specific RNA methyltransferase by catalyzing formation of N6,2'-O-dimethyladenosine (m6A(m on internal positions of U2 small nuclear RNA (snRNA: methylates the 6th position of adenine residues with a pre-deposited 2'-O-methylation. Internal m6A(m methylation of snRNAs regulates RNA splicing. Also able to act as a N(6-adenine-specific DNA methyltransferase by mediating methylation of DNA on the 6th position of adenine (N(6-methyladenosine. The existence of N(6-methyladenosine (m6A on DNA is however unclear in mammals, and additional evidences are required to confirm the role of the N(6-adenine-specific DNA methyltransferase activity of METTL4 in vivo. Acts as a regulator of mitochondrial transcript levels and mitochondrial DNA (mtDNA copy number by mediating mtDNA N(6-methylation: m6A on mtDNA reduces transcription by repressing TFAM DNA-binding and bending. N(6-methyladenosine deposition by METTL4 regulates Polycomb silencing by triggering ubiquitination and degradation of sensor proteins ASXL1 and MPND, leading to inactivation of the PR-DUB complex and subsequent preservation of Polycomb silencing (By similarity.
Synonyms	FLJ23017; HsT661; MGC117235
Reference Data

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