ATG5 Rabbit Monoclonal Antibody [Clone ID: R05-4F2]

Specifications

Product Data
Clone Name	R05-4F2
Applications	IF, IP, WB
Recommend Dilution	WB: 1/1000-1/5000 ICC/IF: 1/20-1/100 IP: 1/20
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic peptide of human APG5L/ATG5
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 33 kDa; Observed MW: 55 kDa
Gene Name	autophagy related 5
Database Link	Entrez Gene 9474 Human UniProt ID Q9H1Y0
Background	Swiss-Prot Acc.Q9H1Y0.Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
Synonyms	APG5; APG5-LIKE; APG5L; ASP; hAPG5; OTTHUMP00000017824
Reference Data

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