CD8A Rabbit Monoclonal Antibody [Clone ID: R09-8E7]

Specifications

Product Data
Clone Name	R09-8E7
Applications	IHC, WB
Recommend Dilution	WB: 1/1000 IHC: 1/50-1/200
Reactivity	Human, Hamster
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic peptide of human CD8
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 26 kDa; Observed MW: 29 kDa
Gene Name	CD8a molecule
Database Link	Entrez Gene 925 Human UniProt ID P01732
Background	Swiss-Prot Acc.P01732.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
Synonyms	CD8; Leu2; MAL; OTTHUMP00000203721; p32
Reference Data

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