DNMT1 Rabbit Monoclonal Antibody [Clone ID: R05-3K1]

Specifications

Product Data
Clone Name	R05-3K1
Applications	IF, IP, WB
Recommend Dilution	WB: 1/1000 ICC/IF: 1/20-1/100 IP: 1/20
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic peptide of human Dnmt1
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 183 kDa; Observed MW: 183 kDa
Gene Name	DNA (cytosine-5-)-methyltransferase 1
Database Link	Entrez Gene 1786 Human UniProt ID P26358
Background	Swiss-Prot Acc.P26358.Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). Promotes tumor growth (PubMed:24623306).
Synonyms	AIM; CXXC9; DNMT; FLJ16293; M.HsaI; MCMT; MGC104992
Reference Data

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