H3.3A (H3F3A) Rabbit Monoclonal Antibody [Clone ID: R04-5J3]

Specifications

Product Data
Clone Name	R04-5J3
Applications	IHC, WB
Recommend Dilution	WB: 1/1000 IHC: 1/200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic peptide of human Histone H3.3
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 15 kDa; Observed MW: 15 kDa
Gene Name	H3 histone, family 3A
Database Link	Entrez Gene 3020 Human UniProt ID P84243
Background	Swiss-Prot Acc.P84243.Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms	H3.3A; H3.3B; H3F3; MGC87782; MGC87783; OTTHUMP00000035619; OTTHUMP00000035621
Reference Data

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