RPA34 (RPA2) Rabbit Monoclonal Antibody [Clone ID: R02-8A3]

Specifications

Product Data
Clone Name	R02-8A3
Applications	IHC, WB
Recommend Dilution	WB: 1/1000-1/5000 IHC: 1/50-1/500
Reactivity	Human, Rat
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic peptide of human RPA32/RPA2
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 29 kDa; Observed MW: 29 kDa
Gene Name	replication protein A2
Database Link	Entrez Gene 6118 Human UniProt ID P15927
Background	Swiss-Prot Acc.P15927.As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
Synonyms	REPA2; RPA32; RPA34
Reference Data

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