Topoisomerase I (TOP1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF, IHC, WB
Recommend Dilution	WB: 1/1000-1/2000 IHC: 1/50-1/200 ICC/IF: 1/50-1/200 FC: 1/50
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthesized peptide derived from human TOP1
Formulation	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	91kDa
Gene Name	topoisomerase (DNA) I
Database Link	Entrez Gene 7150 Human UniProt ID P11387
Background	Swiss-Prot Acc.P11387.Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand.
Synonyms	TOPI
Reference Data

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