VPS4A Rabbit Monoclonal Antibody [Clone ID: R01-9H9]

Specifications

Product Data
Clone Name	R01-9H9
Applications	IF, IHC, WB
Recommend Dilution	WB: 1/2000-1/10000 IHC: 1/500 ICC/IF: 1/100
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic peptide of human VPS4a
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 49 kDa; Observed MW: 49 kDa
Gene Name	vacuolar protein sorting 4 homolog A
Database Link	Entrez Gene 27183 Human UniProt ID Q9UN37
Background	Swiss-Prot Acc.Q9UN37.Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission (PubMed:24814515). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
Synonyms	FLJ22197; hVPS4; SKD1; SKD1-homolog; SKD1A; SKD2; VPS4; VPS4-1
Reference Data

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