IL2 Receptor alpha (IL2RA) Rabbit Monoclonal Antibody [Clone ID: Basiliximab]

Specifications

Product Data
Clone Name	Basiliximab
Applications	Bl, FC, IF, IHC, WB
Reactivity	Human, Monkey
Host	Rabbit
Clonality	Monoclonal
Immunogen	Basiliximab was prepared by immunizing BALB/c mice with CTC 16 cells.
Specificity	Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit – binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R (αβγc) – T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation. Basiliximab is derived from the murine anti-Tac antibody. For the clinically used format, the variable regions from this antibody were grafted onto the sequence of the human IgG1κ constant regions. Basiliximab binding to CD25 competitively blocks the interaction of IL-2R with its ligand IL-2 on the surface of T cells. Blocking this interaction prevents T cell replication and T cell-mediated B cell activation. Basiliximab binding suppresses the immune system inhibits the pathway activating the cellular immune response to allograft rejection. IL-2 signalling suppression can additionally prevent acute graft-versus-host disease, autoimmune disorders and malignancies such as T cell leukemias (Binder et al, 2007). Basiliximab has also been used in FC, IF, IHC and WB studies, which enables the detection of CD25.
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	interleukin 2 receptor subunit alpha
Database Link	Entrez Gene 3559 Human UniProt ID P01589
Synonyms	CD25; IDDM10; IL-2-RA; IL2-RA; IL2R; p55; TCGFR
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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