EPCAM Rabbit Monoclonal Antibody [Clone ID: HEA125]

Specifications

Product Data
Clone Name	HEA125
Applications	FC, IF, IHC, IP, R, WB
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	This antibody was raised by immunising BALB/c mice with the human colon carcinoma cell line HT-29.
Specificity	This antibody is specific for the extracytoplasmic part of human EpCAM, a stable and widely-distributed marker expressed on the surface of many adenocarcinomas. It reacts with carcinomas derived from the colorectum, stomach, pancreas, liver, lung, mammary gland, ovary, thyroid, kidney, urinary bladder and prostate. This antibody has applications in the detection of normal and neoplastic epithelial cells, and distinguishing carcinomas from non-epithelial neoplasms (Momburg et al, 1987). This antibody has been used in radioimmunoassays on live cells and immunoenzymatic staining assays on glutaraldehyde-fixed cells, as well as in immunoperoxidase staining of frozen and formalin-fixed paraffin tissue sections (Moldenhauer et al, 1987; Momburg et al, 1987). These revealed it to react with most normal human epithelial cells, but not non-epithelial tissue, and to intensely react with human carcinomas and metastatic lesions, but not lymphoma, melanoma, sarcoma or neuroblastoma cell lines (Moldenhauer et al, 1987; Momburg et al, 1987). Squamous cell carcinomas stain less strongly than adenocarcinomas, and keratinizing areas of tumor masses remain negative (Momburg et al, 1987). This antibody has also been used in immunoprecipitation analysis to characterise human EpCAM (Moldenhauer et al, 1987), and in immunohistochemical analysis of EpCAM expression in formalin-fixed, paraffin-embedded liver tissue sections (Igarashi et al, 2013; Wang et al, 2018) and frozen liver tissue sections (Crosby et al, 1998). Additionally, it has been used in the immunocytochemical detection of EpCAM expression in mononuclear cell preparations obtained from human peripheral blood or lamina propria (Qiao et al, 1996).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	epithelial cell adhesion molecule
Database Link	Entrez Gene 4072 Human UniProt ID P16422
Synonyms	17-1A; 323/A3; CD326; CO-17A; CO17-1A; EGP; EGP-2; EGP34; EGP40; EGP314; Ep-CAM; ESA; GA733-2; HEA125; hEGP-2; hEGP314; KS1/4; KSA; Ly74; M1S2; M4S1; MH99; MIC18; MK-1; MOC31; TACST-1; TACSTD1; TROP1
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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