L1CAM Mouse Monoclonal Antibody [Clone ID: L1-14.10]

Specifications

Product Data
Clone Name	L1-14.10
Applications	FC, IF, IHC, IP, WB
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	This antibody was raised by immunising mice with recombinant L1-Fc fusion protein, consisting of the ectodomain of human L1.
Specificity	This antibody is specific for the ectodomain (amino acids M1 to E1123) of human L1, and recognises an epitope in the third Ig domain. L1 is over-expressed in a range of tumours, including neuroblastomas, renal carcinomas, ovarian and endometrial carcinomas, and melanomas. This antibody does not cross-react with human CHL1. The specificity of this antibody has been confirmed in flow cytometric (Schafer et al, 2012; Huszar et al, 2006) and Western blot analysis (Huszar et al, 2006; Wolterink et al, 2010). This antibody has been shown, when incubated with SKOV3ip human ovarian carcinoma cells, to efficiently and sustainedly block extracellular signal-regulated kinase (Erk) phosphorylation (Gast et al, 2008). This antibody has been used in immunohistochemical staining for L1 expression, using paraffin-embedded human ovarian carcinoma tissue sections (Wolterink et al, 2010; Knogler et al, 2007) and a panel of human tumour tissue sections, including benign and malignant tumours of the female genital tract (Huszar et al, 2006), and renal cell carcinoma, schwannoma, gastric conventional GIST, pancreatic neuroendocrine tumor and paraganglioma (Inaguma et al, 2016). This antibody, when administered to SCID mice in combination with cytostatic drugs, efficiently reduces the growth of subcutaneously grown Colo357 or SKOV3ip tumors (Schafer et al, 2012).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	L1 cell adhesion molecule
Database Link	Entrez Gene 3897 Human UniProt ID P32004
Synonyms	CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAML1; OTTHUMP00000025992; OTTHUMP00000063091; S10; SPG1
Reference Data

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