CD43 (SPN) Rabbit Monoclonal Antibody [Clone ID: 84-3C1]

Specifications

Product Data
Clone Name	84-3C1
Applications	FC, IF, IHC, IP, WB
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	This antibody was raised by immunizng mice with stimulated human leukocytes.
Specificity	This antibody recognizes CD43 which is a predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. This antibody can be used in the identification of malignant lymphomas. CD43 is expressed on 22-37% of B-cell lymphomas and, at the same time, it is not expressed on reactive B-cells. Consequently, B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma. If used with an anti-CD20 antibody, this clone may be utilized in the immunophenotyping of the lymphomas in formalin-fixed tissues, as Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 usually indicates lymphoma. Importantly, 84-3C1 antibody can be used for the detection of CD43 via various methods. For instance, it was successful in indetifying that antigen in a lysate of dendritic cells' platellet via Western blot (Julien et al., 2007). Finally, another group used this antibody during flow cytometric analysis a sample of B-cells (Yoon et al., 2017).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	sialophorin
Database Link	Entrez Gene 6693 Human UniProt ID P16150
Synonyms	CD43; Galactoglycoprotein; GALGP; GPL115; leukosialin; LSN; Sialophorin
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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