CD53 Rabbit Monoclonal Antibody [Clone ID: 161-2 (53/2)]

Specifications

Product Data
Clone Name	161-2 (53/2)
Applications	FC, FN, IF
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	This antibody was raised by immunizng mice with stimulated human leukocytes.
Specificity	This antibody recognizes human CD53. It does not react with the baboon and horse antigen. Cd53 is a member of tetraspanin family and is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. This antibody is recommended for the detection and analysis of CD53 via flow cytometry and immunofluorescence. Additionally, one group utilized this antibody for the ligation of the CD53 antigen while researching the potential involvement of tetraspanins (such as CD53) in tumor metastasis (Yunta et al., 2002). Furthermore, CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells, as it is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells.
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	CD53 molecule
Database Link	Entrez Gene 963 Human UniProt ID P19397
Synonyms	MOX44; tetraspanin-25; Tspan-25; TSPAN25
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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