CD63 Rabbit Monoclonal Antibody [Clone ID: NK-1-C3]

Specifications

Product Data
Clone Name	NK-1-C3
Applications	FC, IF, IHC, IP, WB
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Monoclonal
Immunogen	This antibody was raised by immunizing BALB/c mice with a plasma membrane preparation of the human melanoma MeWo cells.
Specificity	This antibody recognizes CD63. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation and plays a role in the activation of cellular signaling cascades. The antibody NK-1-C3 has been widely used for the detection and analysis of CD63 via various methods. For instance, it was utilized as a marker of late endosomes and exosomes and the expression of CD63 was analysed via flow cytometry (Wang et al., 2019). Furthermore, NK-1-C3 (NK1/C3) proved successfull in labelling CD63 in multiple tissues, for example, when localizing CD63 in murine retinal pigment epithelium (RPE)/choroid tissue (Wang et al., 2009). Similarly, this antibody was used to detect CD63 on melanoma cells via immunofluorescence staining (Khazen et al., 2016).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	CD63 molecule
Database Link	Entrez Gene 967 Human UniProt ID P08962
Synonyms	granulophysin; LAMP-3; ME491; MLA1; OMA81H; tetraspanin-30; Tspan-30; TSPAN30
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.