Mesothelin (MSLN) Rabbit Monoclonal Antibody [Clone ID: 11-25]

Specifications

Product Data
Clone Name	11-25
Applications	ELISA, FC, IP
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	The original antibody was generated by intraperitoneally immunizing BALB/c mice with the purified recombinant mesothelin protein.
Specificity	This antibody binds the human mesothelin protein which is highly expressed in many human cancers, including the majority of pancreatic adenocarcinomas, ovarian cancers, and mesotheliomas. This antibody is recommended to be used in combination with mAb IC14-30 for detecting mesothelin with high sensitivity in a capture ELISA. This antibody can also immunoprecipitate recombinant mesothelin. NCI-H226 and NCI-H520 were incubated with anti-MSLN antibodies (11—25 ) or an isotype-matched control antibody and followed by PE-conjugate anti mouse IgG. Antigen expression was detected by flow cytometry (PMID: 18394747). 64Cu labelled 11-25 was used in vitro cell binding assay and in vivo positron emission tomography (PET) imaging in the tumor-bearing mice (PMID: 25883990).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	mesothelin
Database Link	Entrez Gene 10232 Human UniProt ID Q13421
Synonyms	CAK1; mesothelin; MPF; SMR
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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