SPP1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Sandwich ELISA: To detect Human Osteopontin by sandwich ELISA (using 100ul/well antibody solution), a concentration of 0.5 – 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Osteopontin as a detection antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human Osteopontin. Western Blot To detect Human Osteopontin by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human Osteopontin is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Produced from sera of rabbits immunized with highly pure recombinant Human Osteopontin. Anti-Human Osteopontin-specific antibody was purified by affinity chromatography employing an immobilized Human Osteopontin matrix.
Concentration	lot specific
Purification	Osteopontin-specific antibody was purified by affinity chromatography employing an immobilized Human Osteopontin matrix
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Database Link	UniProt ID P10451
Background	Osteopontin is a secreted glycoprotein that functions as a ligand to alphavbeta3 integrin and possibly other receptors. It binds tightly to hydroxyapatite and can act as a structural component of the extracellular mineralized matrix. Osteopontin is initially secreted as a 298 amino acid protein, which is subject to multiple post-translational modifications including glycosylation, phosphorylation, and specific proteolytic cleavages into various smaller molecular weight fragments. Osteopontin is expressed in a wide range of cells and tissues including osteoblasts, various tumor cell lines, extraosseous cells in the inner ear, brain, kidney, deciduum, placenta and odontoblasts. In addition to its involvement in mineralized matrix formation, Osteopontin can also function as a cytokine that stimulates the release of IFNgamma and IL-12, while inhibiting the production of IL-10. Recombinant Human Osteopontin is a 298 amino acid protein, which, due to glycosylation, migrates at an apparent molecular weight of 60.0-65.0 kDa by SDS-PAGE analysis under reducing conditions.
Reference Data

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