AREG Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Sandwich ELISA: To detect Human Amphiregulin by sandwich ELISA (using 100ul/well), a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Amphiregulin as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human Amphiregulin. Western Blot To detect Human Amphiregulin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human Amphiregulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Produced from sera of rabbits immunized with highly pure Recombinant Human Amphiregulin. Anti-Human Amphiregulin-specific antibody was purified by affinity chromatography employing an immobilized Human Amphiregulin matrix.
Concentration	lot specific
Purification	Amphiregulin-specific antibody was purified by affinity chromatography employing an immobilized Human Amphiregulin matrix
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Database Link	UniProt ID P15514
Background	Amphiregulin is an EGF related growth factor that signals through the EGF/TGF-alpha receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, Amphiregulin’s extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. Recombinant Human Amphiregulin is an 11.3 kDa glycoprotein consisting of 98 amino acid residues.
Reference Data

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