DEFB105A Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Sandwich ELISA: To detect hBD-5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human BD-5 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBD-5. Western Blot To detect hBD-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-5. Anti-Human BD-5 specific antibody was purified by affinity chromatography and then biotinylated.
Concentration	lot specific
Purification	BD-5 specific antibody was purified by affinity chromatography and then biotinylated
Conjugation	Biotin
Storage Condition	Store at -20°C as received.
Background	Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, six humanbeta-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. Thebeta-defensin proteins are expressed as the C-terminal portion of precursors, and are released by proteolytic cleavage of a signal sequence and, in some cases, a propeptide sequence. beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant Human BD-5 is a 5.8 kDa protein containing 51 amino acid residues
Reference Data

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