Phospho-Ern1 (pSer724) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:1000 WB Brain: 1:1000
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser724­­­ of mouse IRE1, conjugated to keyhole limpet hemocyanin (KLH).
Specificity	Specific for endogenous levels of the ~130 kDa GluR1 protein phosphorylated at Ser724. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Formulation	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Concentration	lot specific
Purification	Antigen Affinity Purified from Pooled Serum
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	130
Gene Name	endoplasmic reticulum (ER) to nucleus signalling 1
Database Link	Entrez Gene 78943 Mouse UniProt ID O75460
Background	IRE1, inositol requiring 1 protein, is an ER transmembrane sensor that activates unfolded protein response (UPR) to maintain the ER and cellular function (Chen et al, 2013). The activation of UPR involves three signaling pathways, IRE1, PERK, and ATF6, which are crucial to returning protein homoeostasis to levels of non-stressed cells (Stewart et al, 2012). Changes in ER homeostasis causing unfolded protein buildup can be due to Ca2+ depletion, hypoxia, altered glycosylation, and viral infection triggering the UPR and activation of IRE1 (Stewart et al, 2012). UPR dysfunction plays an important role in the pathogenesis of neurodegenerative diseases including Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis and Huntington's disease, which is characterized by the accumulation and aggregation of misfolded proteins (Xiang C et al, 2017). The phosphorylation of IRE1 at ser724 may play a significant role in understanding these diseases.
Synonyms	FLJ30999; hIRE1p; IRE1; Ire1-alpha; IRE1a; IRE1P; MGC163277; MGC163279
Note	Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.