MECP2 Mouse Monoclonal Antibody [Clone ID: 5H12]

Specifications

Product Data
Clone Name	5H12
Applications	ICC, IHC, WB
Recommend Dilution	WB: 1:2000 WB Brain: 1:2000 ICC: 1:1000-1:5000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant MeCP2
Specificity	Specific for endogenous levels of the MeCP2 protein. Due to usually high charge, the protein runs at ~75 kDa in SDS-PAGE of mouse brain lysates.
Formulation	PBS + 50% glycerol and 5 mM NaN3
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	75
Gene Name	methyl-CpG binding protein 2
Database Link	Entrez Gene 4204 Human UniProt ID P51608
Background	MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998). Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999). RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
Synonyms	AUTSX3; DKFZp686A24160; MRX16; MRX79; MRXS13; MRXSL; PPMX; RS; RTS; RTT
Note	Protein G purified culture supernatant
Reference Data

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