Phospho-Pank4 (pThr63) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:1000 WB Brain: 1:1000
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr63 of mouse PanK4, conjugated to keyhole limpet hemocyanin (KLH).
Specificity	Specific for the ~86 kDa PanK4 phosphorylated at Ser63. Immunolabeling of PanK4 is nearly eliminated with λ-phosphatase treatment.
Formulation	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Concentration	lot specific
Purification	Antigen Affinity Purified from Pooled Serum
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	86
Gene Name	pantothenate kinase 4
Database Link	Entrez Gene 269614 Mouse UniProt ID Q80YV4
Background	Pantothenate kinase, PanK, is a vital regulatory enzyme for coenzyme A (CoA) biosynthesis, phosphorylating pantothenate (vitamin B5) to 4’-phosphopantothenate, then quickly transforming to CoA which is an essential component for fatty acid metabolism (Abiko, Y, 1967). There are 4 members of the PanK family, located on chromosomes 10q23.31, 20p13, 5q35, and 1p36.32 (Zhou et al, 2001). PanK1 is predominantly in heart, liver, and kidney. PanK2 is expressed ubiquitously, with higher levels in retinal and infant basal ganglia. PanK3 has high levels in liver, while PanK4 is expressed ubiquitously with its highest levels found in muscle (Zhou et al, 2001). Additionally, PanK4 has been shown to regulate Pkm2 activity affecting glucose metabolism (Li et al, 2005). There have been several phospho-serine, threonine, and tyrosine sites identified within PanK4, the role of each one has yet to be determined.
Synonyms	DKFZp547M242; FLJ10782; hPanK4
Note	Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Reference Data

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