CD55 Mouse Antibody [Clone ID: M033]

Specifications

Product Data
Clone Name	M033
Applications	ICC, IP, WB
Recommend Dilution	WB: 1:1000 ICC: 1:100
Reactivity	Human
Host	Mouse
Immunogen	Clone M033 was generated from a proprietary antigen related to the extracellular region of human CD55 from the MDA-MB-231 breast cancer cell line.
Specificity	Clone M033 was purified using Protein G chromatography. The antibody. The antibody also detects a "native" human recombinant CD55 protein that includes the extracellular region. The antibody can be used for native western blot, immunoprecipitation, protein ELISA, and immunocytochemistry, as well for detecting CD55 in live, unfixed cells.
Formulation	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	80
Database Link	UniProt ID P08174
Background	CD55, also known as Decay-accelerating factor (DAF) is an inhibitor of the complement system, and is broadly expressed in malignant tumours. In cancer, CD55 has been implicated in tumorigenesis, neoangiogenesis, and metastasis. CD55 may decrease complement mediated tumor cell lysis, inhibit tumor apoptosis, and promote invasive cancer cell motility. These roles in cancer may involve binding to the seven-span transmembrane receptor CD97. In neuroblastoma cells, CD55 contributes to growth of colonies and to invasion of cells, but not to stemness. In neuroblastoma cells, CD55 is upregulated in a small population of cells that are HIF-2α positive. This CD55 positive subpopulation is highly invasive and has low adhesion to fibronectin and collagen. In addition, CD55 expression correlates with poor prognosis in neuroblastoma patients.
Note	Protein G purified tissue culture supernatant.
Reference Data

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