L1CAM Mouse Antibody [Clone ID: M023]

Specifications

Product Data
Clone Name	M023
Applications	ICC, IP, WB
Recommend Dilution	WB: 1:1000 ICC: 1:100
Reactivity	Human
Host	Mouse
Immunogen	Clone (M023) was generated from a proprietary antigen related to the native human L1CAM expressed in the MeWo melanoma cell line.
Specificity	Clone M023 mouse monoclonal antibody detects a 230 kDa* protein on SDS-PAGE immunoblots of human MeWo, MDA-MB-231, A431, and MCF7 cells, as well as human brain tissue. The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects L1CAM on live cells.
Formulation	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	230
Database Link	UniProt ID P32004
Background	The basal lamina contains Collagen Type IV, proteoglycans, and glycoproteins. Laminin is a high molecular weight (850 kDa) oligomer, consisting of three different chains (α, β, and γ) joined by disulfide bonds. The structure of laminins include two helical domains (I & II) at the COOH-terminal, a laminin IV domain, multiple EGF-like repeats, and a laminin globular domain (G), as well as an N-terminal domain VI. Domains IV and VI are the binding sites for collagen and heparan sulfate, respectively. Several isoforms have been identified for the genes of each chain. Laminin γ1 (laminin B2) contains 14 glycosylation sites and 12 cysteine repeat domains. The expression of the Laminin subunits is found in the basal lamina of tissues. Here, the protein interacts with other extracellular matrix components to mediate cell attachment, migration and organization during embryonic development.
Note	Protein G purified tissue culture supernatant.
Reference Data

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