JNK1 (MAPK8) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1:10000~1:20000 IHC: 1:50~1:200 IF: 1:50~1:200
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phosphopeptide derived from human JNK1/2/3 around the phosphorylation site of Threonine 183 / Tyrosine 185.
Specificity	JNK1/2/3 (Phospho-T183/Y185) polyclonal antibody detects endogenous levels of JNK1/2/3 protein only when phosphorylated at Thr183/Tyr185.
Formulation	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Concentration	1mg/ml
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Predicted Protein Size	~ 54 kDa
Gene Name	mitogen-activated protein kinase 8
Database Link	Entrez Gene 5599 Human UniProt ID P45983
Background	The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists. As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase. Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs. Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member. There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms. SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors.
Synonyms	c-Jun N-terminal kinase 1; JNK-46; JNK1, PRKM8, SAPK1, SAPK1C; MAPK 8; MAPK8; MAP kinase 8; Mitogen-activated protein kinase 8; SAPK1c; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1
Note	For research use only, not for use in diagnostic procedure.
Reference Data

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