MPRIP Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:5000~1:10000 IP:1:2000~1:8000 IHC:1:50~1:100
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant protein of human M-RIP.
Specificity	M-RIP polyclonal antibody detects endogenous levels of M-RIP protein.
Formulation	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Concentration	1mg/ml
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Predicted Protein Size	~ 130-160kDa
Gene Name	myosin phosphatase Rho interacting protein
Database Link	Entrez Gene 23164 Human UniProt ID Q6WCQ1
Background	Myosin phosphatase-rho interacting protein (M-RIP), also known as p116RIP, RIP3, and MPRIP, localizes to actin-myosin filaments regulating cytoskeletal dynamics. M-RIP contains amino-terminal pleckstrin homology domains, carboxyl-terminal coiled-coil domains, and was originally identified to associate with the myosin phosphatase complex. M-RIP binds to MBS/MYRT, the myosin binding subunit of myosin phosphatase, as well as RhoA. Phosphorylation of MYRT by Rho-associated kinase (ROCK) inhibits myosin phosphatase activity, resulting in increased levels of phosphorylation on myosin light chain, and enhanced contractility. M-RIP may function as a scaffolding protein for the complex between the myosin phosphatase complex, Rho/ROCK, and actin. Silencing of M-RIP results in disassembly of the complex, increased phosphorylation of myosin light chain, and changes to cytoskeletal dynamics.
Synonyms	M-RIP; MPRIP; Myosin phosphatase Rho-interacting protein; p116Rip; Rho-interacting protein 3; RIP3
Note	For research use only, not for use in diagnostic procedure.
Reference Data

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