MASTL Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:500~1:1000
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 820-880 of Human MASTL.
Specificity	MASTL (F850) polyclonal antibody detects endogenous levels of MASTL protein.
Formulation	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration	1mg/ml
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Predicted Protein Size	~ 110 kDa
Gene Name	microtubule associated serine/threonine kinase like
Database Link	Entrez Gene 84930 Human UniProt ID Q96GX5
Background	Mitotic control is important for normal growth, development, and maintenance of all eukaryotic cells. Research studies have demonstrated that inappropriate control of mitosis can lead to genomic instability and cancer. A regulator of mitosis, Greatwall kinase (Gwl), was first identified in Drosophila melanogaster. Subsequent studies showed that, based on sequence homology and function, microtubule-associated serine/threonine kinase-like (MASTL) is the human ortholog of Gwl. Regulation of MASTL/Gwl activation has been shown to be critical for the correct timing of mitosis. Research studies have shown that Gwl is activated by hyperphosphorylation. The phosphorylation of human Gwl at Thr194 and Thr207 by active cyclin B1-cdc2 leads to possible autophosphorylation at Ser875 (Ser883 in Xenopus), which stabilizes the kinase. Activated Gwl phosphorylates α-Endosulfine (ENSA) and cAMphospho-regulated phosphoprotein 19 (ARPP19) at Ser67 and Ser62, respectively. Phosphorylated ENSA and ARPP19 inhibit the activity of the B55 subunit-associated form of protein phosphatase 2A (PP2A-B55), allowing for complete phosphorylation of mitotic substrates by cyclin B1-cdc2 and mitotic entry. When Gwl is inactivated, PP2A-B55 reactivates, which leads to dephosphorylation of cyclin B1-cdc2 and mitotic exit.
Synonyms	GW; GWL; hGWL; MAST-L; MASTL; Microtubule-associated serine/threonine-protein kinase-like; Serine/threonine-protein kinase greatwall; THC2
Note	For research use only, not for use in diagnostic procedure.
Reference Data

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