ASF1A Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:1000~1:2000
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 120-180 of Human Asf1a.
Specificity	Asf1a (H150) pAb detects endogenous levels of Asf1a protein.
Formulation	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration	1mg/ml
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Predicted Protein Size	~ 22 kDa
Gene Name	anti-silencing function 1A histone chaperone
Database Link	Entrez Gene 25842 Human UniProt ID Q9Y294
Background	ASF1 was first identified in S. cerevisiae based on its ability to de-repress transcriptional silencing when overexpressed. While only one gene exists in yeast and Drosophila, mammalian cells contain the two highly homologous ASF1A and ASF1B genes. ASF1A and ASF1B function as histone chaperones, delivering histone H3/H4 dimers to CAF-1 or HIRA histone deposition complexes to facilitate replication-coupled and replication-independent nucleosome assembly on DNA. Both ASF1A and ASF1B bind to CAF-1, but only ASF1A binds to HIRA. In addition to playing a role in DNA replication and gene silencing, ASF1 functions in DNA damage repair, genome stability and cellular senescence. Deletion of ASF1 in yeast and Drosophila confers sensitivity to various DNA damaging agents and inhibitors of DNA replication, increases genomic instability and sister chromatid exchange, and activates the DNA damage checkpoint. Depletion of both ASF1A and ASF1B in mammalian cells results in the accumulation of cells in S phase, increased phosphorylation of H2A.X, centrosome amplification and apoptosis. ASF1A is required for the formation of senescence-associated heterochromatin foci (SAHF), with overexpression of ASF1A inducing senescence in primary cells. Both ASF1A and ASF1B are phosphorylated in S phase by the Tousled-like kinases TLK1 and TLK2, and are dephosphorylated when TLK1 and TLK2 are inactivated by Chk1 kinase in response to replicative stress. The function of ASF1 phosphorylation is not yet understood.
Synonyms	Anti-silencing function protein 1 homolog A; ASF1A; CCG1-interacting factor A; CGI-98; CIA; hAsf1; hAsf1a; hCIA; Histone chaperone ASF1A; HSPC146
Note	For research use only, not for use in diagnostic procedure.
Reference Data

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