IRS1 Rabbit Polyclonal Antibody
CAT#: TA392915
IRS-1 (phospho-S323) polyclonal antibody
Size: 25 ul
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Specifications
Product Data | |
Applications | WB |
Recommend Dilution | WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of Serine 323. |
Specificity | p-IRS-1 (S323) polyclonal antibody detects endogenous levels of IRS-1 only when phosphorylated at Ser323. |
Formulation | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
Concentration | 1mg/ml |
Conjugation | Unconjugated |
Storage Condition | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
Predicted Protein Size | ~ 132, 180 kDa |
Gene Name | insulin receptor substrate 1 |
Database Link | |
Background | IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor. |
Synonyms | Insulin receptor substrate 1; IRS-1; IRS1 |
Note | For research use only, not for use in diagnostic procedure. |
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