Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:500 - 1:1000 ELISA: 1:1000 - 1:2000
Reactivity	Escherichia coli
Host	Rabbit
Clonality	Polyclonal
Immunogen	Anti-Host Cell Proteins Antibody was prepared blending antibodies that have been separately developed by repeated immunizations with a mixture of high molecular weight (HMW) and a mixture of low molecular weight (LMW) proteins from E.Coli (BL21).
Specificity	E.coli HCP Antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins. This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. The anti-sera reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	77.5 mg/mL - lot specific
Conjugation	Unconjugated
Storage Condition	Store Host Cell Proteins Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Background	Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
Synonyms	rabbit anti-E.Coli Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, ECP antibody, Anti-ECP, 2D detection antibody
Note	Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and has been tested in ELISA, 2D-Page and Western blot.
Reference Data

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